Reporter

Part:BBa_K3675002:Design

Designed by: João Costa   Group: iGEM20_NOVA_LxPortugal   (2020-10-22)


GFPuv


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 523
    Illegal XhoI site found at 424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence possesses a codon optimized for expression in a mammalian host. We decided not to remove it as it can be used by more teams in the future.


Source

The source for this sequence is the cloning vector pBAD-GFPuv whose authors are:Crameri,A., Whitehorn,E.A., Tate,E. and Stemmer,W.P. The original paper " Improved green fluorescent protein by molecular evolution using DNA shuffling" can be found under pubmed ID 9630892.

References